Product Name :
RNF43 Rabbit Polyclonal Antibody
Predicted band size :
Observed band size :
Synonyms:
RNF124 Ring finger protein 43 URCC RNF43_HUMAN E3 ubiquitin-protein ligase RNF43 RING finger protein 43.
Function :
Ring finger protein 43 is a protein that in humans is encoded by the RNF43 gene. The protein encoded by this gene is a RING-type E3 ubiquitin ligase and is predicted to contain a transmembrane domain, a protease-associated domain, an ectodomain, and a cytoplasmic RING domain. This protein is thought to negatively regulate Wnt signaling, and expression of this gene results in an increase in ubiquitination of frizzled receptors, an alteration in their subcellular distribution, resulting in reduced surface levels of these receptors. Mutations in this gene have been reported in multiple tumor cells, including colorectal and endometrial cancers. Alternative splicing results in multiple transcript variants encoding different isoforms.
Antibody Type:
Rabbit Polyclonal Antibody
Immunogen :
KLH conjugated synthetic peptide derived from human RNF43 41-140/783
Species reactivity:
Human, Mouse, Rat, Dog, Cow, Horse, Rabbit
Verify application:
WB, ELISA, IHC-P, IF
Coupling :
unconjugated
Clone number:
RRID:
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at -20℃ for one year.247592-95-6 Purity Avoid repeated freeze/thaw cycles.3-Amino-5-chloropyrazine-2-carbaldehyde structure The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃.PMID:33394079 When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage buffer solution:
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
83 KDa
Positive control :
Expressed in fetal kidney, fetal lung, in colon cancer tissues, hepatocellular carcinomas and lung adenocarcinomas. Overexpressed in colorectal cancer cell lines.